Development
of Integrated Solanum bulbocastanum Genetic and Physical Maps as a Communal
Resource for Mapping and Isolation of R Genes
Aversano, R., E.A. Quirin, R.L. Syverson, D.
Carputo, and J.M. Bradeen
Plant
& Animal Genome XIII (
Primitive potatoes, including the
Mexican diploid Solanum bulbocastanum, are noteworthy sources of
broad-spectrum resistance to several pathogens. We are developing integrated
resources of genetic and resistance (R) gene physical maps for the primitive potatoes
using Solanum bulbocastanum as a model. An AFLP linkage map is being
constructed. We have generated fragments from 13 previously cloned R genes and
are generating R gene analogs using degenerate PCR. BACs corresponding to R
gene fragments will be isolated, assembled into contigs and integrated into the
genetic map. BAC end sequences for each contig will serve as a marker
development resource for other potato species. We are testing the utility of
PCR markers developed from S. bulbocastanum R gene contig sequence in tomato,
cultivated potato, eggplant, and other Solanum species. Given documented
synteny in the genus Solanum, we seek to answer whether R genes map to
common genome locations in tomato, potato, and S. bulbocastanum.
Furthermore, the integrated mapping resource will aid future R gene mapping and
cloning efforts.